The PanProbes™ One-Step RT-qPCR Kit UNG is a one-step multiplex qRT-PCR test cassette. This kit has been specially optimized to effectively complete 1-4 qRT-PCR tests with high sensitivity and specificity. During the experiment, cDNA synthesis and qPCR reactions were done in the same reaction system, simplifying the experimental operation and reducing the risk of contamination. In addition, the dUTP/Heat-sensitive UNG anti-contamination system is used in this kit to effectively prevent gas-soluble contamination, and the heat-sensitive UNG can be quickly inactivated during reverse transcription, ensuring the expansion efficiency of qRT-PCR. This product is suitable for multiplex detection of RNA viruses and trace RNA samples, with sensitivity up to 1 pg of total RNA or < 10 copies of RNA samples.

• Performance

Figure 1. Sensitivity performance of QPR02
The amplification performance and sensitivity of QPR02 were evaluated by qPCR using mouse RNA templates (100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg). The amplification curves demonstrate excellent amplification performance and sensitivity, with the lowest detectable template amount being 1 pg.

Figure 2. Testing of QPR02 with different concentrations of COVID-19 sequence templates (A) Comparison of QPR02 with competitor V (B) Comparison of QPR02 with competitor M
Amplification of different concentrations of COVID-19 sequence templates using QPR02 was performed by qPCR, with fluorescence signals collected for FAM, VIC, and Cy5. The amplification curves indicate that the amplification performance of QPR02 is comparable to that of two competitors V. Moreover, the amplification curves and fluorescence intensity of QPR02 are superior to those of competitor M.