This product is a new contamination-proof, dye-based real-time PCR master mix developed, based on the PanProbes™ Universal qPCR MasterMix (Cat. QPD01-0100), by adding an optimized ratio of dUTP and UNG enzymes. It contains optimized concentrations of the Hotstart DNA polymerase, dNTPs, dUTP, UNG enzyme (uracil DNA glycosylase), Mg2+, reaction buffer, and stabilizer. In the PCR reaction, dUTP is used instead of dTTP, and the T in the amplification product fragment is replaced by the U to form a PCR amplification product containing dU bases, and the highly active UNG enzyme can quickly degrade the U-containing DNA fragment in the reaction system, effectively eliminating the residual contamination of PCR products in the environment and greatly reducing the false positive caused by the amplification product contamination, thus ensuring the specificity and accuracy of the amplification. This product is a 2× contamination-proof, probe-based real-time PCR premix reaction system, only requiring to add the template, primers, probe, ROX Reference Dye (used according to different real-time PCR instruments) and water to make its working concentration 1× for carrying out the reaction. It has the advantages of rapidness and simplicity, high sensitivity, strong specificity, and good stability, which can minimize the human error, save PCR experimental operation time, and reduce the probability of contamination.

• Performance

Figure 1. Detection Test of QPD02 on Different Genes
Using QPD02 for reverse transcription on serially diluted Swine RNA templates, followed by qPCR, the amplification curve showed efficient and sensitive amplification of different genes.