This product is a premixed reagent for the first-strand cDNA synthesis developed based on the RScript II Reverse Transcriptase. RScript II Reverse Transcriptase has a higher thermal stability than the RScript Reverse Transcriptase. It can optimize the reverse transcription temperature to 55-60°C for complex secondary structures and high GC content targets, overcome the inhibition of cDNA synthesis by complex RNA secondary structures, and effectively synthesize the high-quality cDNA. RScript II Reverse Transcriptase has a higher cDNA synthesis capacity, making it ideal for the reverse transcription of small amounts of template as well as low-copy genes. This product is provided conveniently in the form of premixed reagents - 2× Sharp Buffer (with the primers) containing an optimized buffer system, dNTPs, and premix of the random primer and Oligo18 (dT).  RScript II RT Mix contains the ratio-optimized RScript II Reverse Transcriptase and RNase inhibitor, enabling the synthesis of the first-stranded cDNA from very low amounts of total RNA or poly(A) mRNA. The synthesized single-stranded cDNA product can be used directly for subsequent PCR or qPCR reactions.

• Performance

Figure 1. qPCR Results Using RK002-0050 with Different Concentrations of Hela RNA as Templates
Hela RNA templates were serially diluted to 100 ng, 10 ng, 1 ng, 100 pg, 10 pg. cDNA obtained from reverse transcription was subjected to qPCR, demonstrating excellent amplification efficiency and linear expression.