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A novel way to perform Western Blot
Mix OneStep Blocker, 1°Ab and 2° Ab with membrane together, that's all.
OneStep Blocker is a blocking solution for Western blot analysis.
This OneStep Blocker buffer not only provides blocking, primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the 3-in-1 step procedure, OneStep Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.