10X Concentrate Ponceaus S Protein Staining Solution is a membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S Protein staining is reversible and can be removed with a short incubation in 0.1% NaOH.

Figure 1. Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. The PVDF membrane stained Ponceau S for 5 mins and washed with deionized water for 3 mins. It provides visible pink bands.