Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.

Figure 1 Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. The PVDF membrane stained Ponceau S for 5 mins and washed with ddH₂O for 3 mins. It provides visible pink bands.

Lane 1, 13: Prestained Protein Ladder #PM019-0500

Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500

Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA